منابع مشابه
Establishment of a Primary Cell Culture of Human Fibroblast in Iran
Background: Human fibroblasts are the part of the dermis that secrete extracellular matrix for the purpose of tissue repair. Culturing fibroblasts, which leads to formation of a monolayer of these cells, is used for treating various conditions including thermal burns and other skin defects such as diabetic and varicose vein leg ulcers. Therefore, we aimed at developing a fibroblast bank to acco...
متن کاملPellet culture model for human primary osteoblasts.
In vitro monolayer culture of human primary osteoblasts (hOBs) often shows unsatisfactory results for extracellular matrix deposition, maturation and calcification. Nevertheless, monolayer culture is still the method of choice for in vitro differentiation of primary osteoblasts. We believe that the delay in mature ECM production by the monolayer cultured osteoblasts is determined by their state...
متن کاملInduction of CYP2C genes in human hepatocytes in primary culture.
The expression and inducibility of four CYP2C genes, including CYP2C8, -2C9, -2C18, and -2C19, was investigated in primary cultures of human hepatocytes. By the use of RNase protection assay and specific antibodies, each CYP2C mRNA and protein were quantified unequivocally. The four CYP2C mRNAs were expressed in human livers and cultured primary hepatocytes, but only the CYP2C18 protein was not...
متن کاملSenescence of human vocal fold fibroblasts in primary culture
This study examined the replicative senescence of primary-culture human vocal fold fibroblasts, in terms of changes in gross chromosomal structure with subculturing, and population doubling time (PDT). The mRNA expressions of 14 target genes were also examined. The objectives were to identify the onset of senescence for establishing the acceptable limit of sub-culturing, and to better understan...
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Epithelial ovarian cancer is a leading cause of female cancer mortality in the United States. In contrast to other women-specific cancers, like breast and uterine carcinomas, where death rates have fallen in recent years, ovarian cancer cure rates have remained relatively unchanged over the past two decades (1). This is largely due to the lack of appropriate screening tools for detection of ear...
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ژورنال
عنوان ژورنال: The Journal of the Japanese Society of Clinical Cytology
سال: 1986
ISSN: 1882-7233,0387-1193
DOI: 10.5795/jjscc.25.697